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Chemistry deals with lots of concepts ranging from simple to more complex as well as concrete to abstract. Understanding of chemistry is needed mainly by pre-service science teachers. The purpose of this study was to investigate the understanding of preservice science teachers about chemical multiple representations at macroscopic, symbolic, and sub-microscopic levels. This study involved twenty-seven preservice science and data were collected using chemical multiple representations table. Results indicated that most of pre-service science teachers could not describe the substances when it is dissolved in water at macroscopic, symbolic, and sub-microscopic levels. A few of pre-service science teachers couldnt write correctly the symbolic level of potassium chloride. All of them couldn-t write correctly the ionization reaction of acetic acid and also describe the molecular view of acetic acid solution. Generally, they had some difficulties in investigating macroscopic, symbolic, and sub-microscopic levels nor integrating these levels with the contents.