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The need for antioxidant drinks made from herbs in the community increases due to the increasing number of types diseases caused by free radicals. One type of potential herbs as antioxidant is corn silk, in the form of corn silk herbal drinks tea. The basic ingredients for making herbal tea drinks is the dried corn silk. However, the temperature factor and drying time will affect the characteristics of herbal tea drinks produced. The purpose of this study was to determine the effect of temperature and drying time on the content of bioactive compounds (total phenolics and flavonoids) and antioxidant activity of corn silk herbal tea by vacuum drying method. The stages of this study were sample preparation (sorted, washed and dried), then dried the corn silk using a vacuum dryer with 3 temperature variations (40°C, 50°C and 60°C) and 3 drying time variations (3 hours, 4 hours and 5 hours), and finally the brewing process. Samples then analyzed the bioactive compounds (total phenolics, flavonoids) and antioxidant activity. Based on the results of study, the higher of drying temperature resulted on higher phenolics, flavonoids and antioxidant activity. Conversely, the longer of drying time made the total phenolics, flavonoids and antioxidant activity tend to decrease. The best treatment in this study was found in the treatment of drying temperature on 60°C with 4 hours drying time with total phenolics of 11.68 mg GAE/g, flavonoids of 6.47 mg QE/g and antioxidant activity with IC50 values of 278.1 ppm.