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Background: The rate of the microbial exposure incidence among nurses through a variety of ways remains high. Paramedics play a vital role as a medium of nosocomial infection transmission in hospitals, especially the spread through direct contact. The purpose of this research was to analize of the effectiveness of education of MRSA infection prevention and control using directive discourse with improve compliance hand washing among nurses in hospitals. Methods: This was quasi-experimental research using a pretest-posttest non-control design. The research subject consisted of a group of 25 nurses. This research has obtained the certificate of ethical approval issued by the Hospital Ethical Committee. Results: To determine the results of measuring hand washing before and after the treatment, Wilcoxon test was undertaken to these data and the value of p = 0.018 (p < 0.05), meaning that there is a difference in the compliance with hand washing before and after the treatment. The increased compliance was also resulted from the increased awareness relating to the importance of hand washing. Conclusions: The auditing guidelines for MRSA infection prevention and control using directive discourse were effective for improving compliance with hand washing among nurses in hospitals.