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This research aims to analyze the effect of Demonstration - Practical work1 - Discuss - Practical Work2 - Explain (DPDPE) strategy on the quantitative literacy of biology students using photosynthetic kits. This research was carried out with the one group pretest-posttest research design involved 22 biology students at one of the State University in Bandung in the fourth semester of the 2018-2019 academic year. The research instrument used is 10 quantitative literacy test given to students, the indicators consist of interpretation, representation, analysis/application, calculation, assumption, and communication. Data analysis was carried out by recapitulating scores of the pretest and posttest. Analysis of the results in this research data was analyzed with scoring and normalize gain of the score. The results of this research showed increased students quantitative literacy. Based on these results highest enhance after implementation of DPDPE strategy is calculation ability. In general, the results of the study are indicated by the acquisition 0.86 of N-gain averages which is a high category. The application of DPDPE learning strategies using photosynthetic kits can enhance quantitative literacy in biology students.