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Peristaltic pump RPM counter using rotary encoder has been designed. Peristaltic pump is a pump which using flexible tube as a tool for transporting fluid due to the pressure roller on the rotor. The rate of fluid flow in the flexible tube is affected by the angular speed of the motor (ω) in RPM. There are many available peristaltic pumps with a various selection in the market but rarely equipped with RPM measurement system. In this research, the peristaltic pump used three rollers and a flexible tube with the inner radius (rd) 0.21cm. The two main part on the rotary encoder are encoder disk with an index of 36 and a sensor optocoupler chip. Output rotary encoder signal is processed in such a way on the microcontroller into the value of angular speed (ω) in RPM and the linear velocity (v) in cm / min. Then, the results of this calculation is displayed on the LCD. The operating voltage range of peristaltic pumps which can be used are 1.5 to 9.0 V. At this voltage range, the peristaltic pump produce angular speed in a range of 3.76-33.83 RPM. RPM counter system of the peristaltic pump which has been made could measure RPM well with a percentage of the average accuracy percentage of 99.51% and average error percentage of 0.49% of the value of RPM results of manual calculations. The volume flow rate which has been calculated was 8.9407 – 65.0959 ml/min and the volume flow rate which has been measured was 37.1518 – 66.5199 ml/min.