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This research purpose to remove copper heavy metal (Cu2+) with a biotechnological approach using mixed microalgae Chlorella sorokiniana, Monoraphidium sp. and Scenedesmus obliquus which is bound in Na-Alginate polymer. The effect of contact time and temperature on the biosorption rate was examined to achieve the greatest efficiency in the Cu2+ metal removal process. Bonded biosorbents are made by drying in an oven, grinding, and trapping into polymer alginate to form immobilized microalgae or called beads. The decrease in Cu concentration was analyzed using Atomic Absorption Spectroscopy (AAS). The absorption properties of biosorbents were characterized using Fourrier Transform Infrared (FTIR) and Scaning Electron Microscopy (SEM). Optimization of absorption parameters was carried out in a batch system using a concentration of 20 mg/L Cu wastewater, biosorbent concentration 0.5 g/g, pH 4 with contact time setting (60, 120, 180) minutes and temperature regulation (25, 30, 35)oC. The results showed that maximum biosorption occurred at pH 4, temperature 30oC and contact time 120 minutes with removal efficiency 82%. This research prove that immobilized microalgae is very effective for reducing Cu2+ in wastewater, and immobilized microalgae could act as biosorbents which are effective in further optimization.