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This research intends to analyze the mediating role of information acquisition on the linkage of migratory culture toward entrepreneurship. The research population are migrant Madurese entrepreneurs in East Java, Indonesia, with a sampling technique using a proportional random sampling technique with three locations: Surabaya City, Malang City and Jember Regency with 170 Madurese migrant respondents. The consideration is that there are relatively many Madurese in the city. This research was started by designing questionnaires and then validity dan reability test of the research instrument. Further, the questionnaire was broadcasted to Madura migrant entreprenuers who were randomly selected. The primary data analysis technique uses structural equation model with the Partial Least Square (PLS) data processing program. The research finding demonstrates that information acquisition significantly mediates on the linkage of migratory culture toward entrepreneurship. It means that information acquisation plays a significant role to encourage entrepreneurship.