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Optimization is a method of finding variable values that are considered optimal, effective and efficient to achieve the desired results. The experiments were conducted to determine the relationship between response variable and independent variable and the effect of independent variables to response variable. Multiple comparison tests can be used to find the level that makes optimal response. The purpose of this study was to apply the surface response method (Central Composite Design) to obtain optimum process variable conditions in the manufacture of biodegradable plastics. The fix variable in this study were 10 grams of tapioca starch, gelatinization of temperature 69-79oC and total mixture weight (base) consisting of starch, poly (NIPAM)-chitosan, citronella oil, 2% acetic acid and water (100 grams). While for the independent variable is composition of chitosan (0.35%; 0.45%; and 0.55% of total weight), composition of glycerol (1.5%; 2.5%; and 3.5% of total weight) and composition of lemongrass oil (0.25%; 0.35% and 0.45% of total weight). The response variable is tensile strenght and break of elongation for each biodegradable plastic sample made. The application of this response surface method can be used to obtain an independent variable that makes the optimal response variable.