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In vitro antibacterial assay of Camellia sinensis leaf extract and Cotrimoxazole on Staphylococcus aureus
Dewi Wahidatul Khasanah, Reza Hakim, Erna Sulistyowati

University of Islam Malang (UNISMA)


Abstract

Background: Green tea leaf (Camelia sinensis) possess certain antibacterial compounds. Recent studies have been presented that C sinensis has critical roles in bacterial breakdown proccess. This study identified the antibacterial activity of C. sinensis leaf extract against Staphylococcus aureus in combination with cotrimoxazole. Methods: Methanolic or decoction extract of C sinensis were determined. The antibacterial activities (single or in combination with cotrimoxazole) were measured with zone of inhibition (ZOI) assay through agar well diffusion method. These ZOI were evaluated with Ameri-Ziaei Double Antibiotic Synergism Test (AZDAST). The data was analyzed with Kolmogorov-Smirnov, followed by a non-parametric Mann-Whitney Test. Significant level was at p<0.05. Results: The ZOI-s diameter of S. aureus on combination of C. sinensis methanolic extract with cotrimoxazole, C. sinensis decoction with cotrimoxazole, low dose of cotrimoxazole, single high dose of C. sinensis methanolic extract, single low dose of C. sinensis methanolic extract, and C.sinensis decoction were 25.67 ± 5.8 mm, 25.67 ± 1.53 mm, 10.33±0.58 mm, 9.67±0.58 mm, 8.67±0.58 mm, and 0 mm, respectively. Combination of C. sinensis methanolic extract with cotrimoxazole and C. sinensis decoction with cotrimoxazole were significantly increased ZOI-s diameter (p<0.05). Combination of C. sinensis methanolic extract and cotrimoxazole has synergistic effect, whereas C. sinensis decoction with cotrimoxazole showed potentiation effect. Conclusion: Combination of C. sinensis leaf extract and cotrimoxazole is potential as an antibacterial agent.

Keywords: Camellia sinensis, Cotrimoxazole, Staphylococcus aureus, antibacterial assay

Topic: Biomedical Science

Link: https://ifory.id/abstract/Bd9bMR4nq8Gm

Conference: International Conference on Life Sciences and Technology (ICoLiST 2019)

Plain Format | Corresponding Author (Erna Sulistyowati)

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