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The purpose of this study was to obtain the right time for pruning and best urine treatment intervals for the growth and yield of melon plants. This research is a factorial experiment, with the basic design of Randomized Block Design (RBD) with 2 factors. This experiment was carried out in a greenhouse from June to September 2018. The interaction between setting the pruning time (P) and giving rabbit urine (U) to the growth and yield of melon plants on all influential variables was not significant. The highest weight obtained from the weight of fruit per plant because the setting of shoot trimming time is given at P1 of 1,555.21 g or increased by 10.12% compared to the lowest yield at P3 which is 1412.34 g. Fruit weight per plant in U1 urine administration with a given interval of 3 days showed the highest value of 1568.88 g or increased by 14.67% compared to the lowest result at U0 which was 1368.17 g.