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In this study, green synthesis AgNP was performed using Diplazium esculentum aqueous extract and characterized using Ultraviolet-visible Spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and transmission electron microscope. The formation of colloidal AgNPs was proven by the significant surface plasmon resonance band at 450 nm and XRD pattern and HRTEM images confirmed the consistent pattern of elliptical crystalline AgNPs in a face-centered cubic (fcc) geometry with an average particle size of 23.385 ± 8.349 nm. FTIR spectral analysis confirmed the presence of biomolecules from the plant extract on the AgNPs acting as capping agent. Catalytic reduction activity showed up to 91% decolourisation of methyl blue obeying a pseudo-first order reaction with a rate constant of 0.0395 min^(-1). For adsorption capacity, 87% adsorption of methylene blue dye was obtained when 3 mg of AgNPs was used. Furthermore, the antibacterial assay exhibited positive results when conducted against Gram-negative bacteria (Escherichia coli ATCC 11229) and Gram-positive bacteria (Staphylococcus aureus ATCC 6538) through disk diffusion technique. Therefore, the biosynthesized AgNPs using Diplazium esculentum aqueous extract is proven active in the removal of the dye via catalytic reduction and adsorption with good potential as antibacterial agent.