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The purpose of this study was to get one of the right time to trim the shoots and giving the urine more often given will be able to give effect to the growth and yield of melon plants both the best quality and quantity as well as the increasing income of melon farmers. This research is a factorial experiment, with a randomized block design (RCBD) basic design with 2 factors tried and carried out in a greenhouse from June to September 2018. In the first factor, shoot pruning consists of 3 levels: P 1 = pruning shoot 7 days, P 2 = pruning 14 days, P 3 = pruning buds 21 days. The second factor with 4 levels of giving rabbit urine: U 0 = without being given urine, U 1 = giving urine every 3 days, U 2 = giving urine once every 6 days, U 3 = giving urine once every 9 days. From the experiment there will be 12 combination treatments and will be repeated 3 times so that there are a total of 36 combination treatments. The data obtained were analyzed by analysis of variance and continued with a 5% BNT or Duncan distance test. The interaction (P x U) between setting the shoot pruning time (P) and giving rabbit (U) urine to the growth and yield of melon (Cucumis melo L.) plants on all influential variables was not significant (P≥0.05). Fruit weight per plant due to setting the shoot pruning time gave the highest weight obtained at P 1 of 1555.21 g which was not significant with P 2 having a weight of 1452.81 g and significantly different from P 3 which was 1412.34 g. The treatment of P 1 each increased by 7.05% and 10.12% against P 2 and P 3. The results of this study showed that the highest fruit weight was obtained when pruning 7 days after planting (P1) supported by 256 plant height, 75 cm, number of lateral shoots 16.92 pieces, number of leaves 80.42 strands, leaf area 14242.72 cm2, leaf wet weight 376.29 g, wet weight of stem and lateral shoots 190.22 g, root wet weight 19.89 g, leaf dry weight 133.03 g, root dry weight 15.36 g, fruit dry weight 298.03 g. Fruit weight per plant on U 1 urine administration with the interval of giving once every 3 days showed the highest value of 1568.88 g different not significant with U 2 and U 3 each of 1482.42 g 1474.36 g. U1 treatment when compared with U 0 without urine administration was significantly different with a value of 1368.17 g, an increase of 14.67%. The high weight of fruit treated urine administration time once every 3 days (U 1) supported by variable plant height 265.17 cm, number of lateral shoots 17.44 pieces, number of leaves 87.88 strands, leaf area 15,590.13 cm2, wet weight of leaves 399 11 g, the wet weight of the stem and lateral shoots 201.71 g, root wet weight 21.66 g, root dry weight 16.02 g, fruit dry weight 322.28 g in treatment U 1 and all had significant effect with maximum value.