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Bamboo fibers have advantages of renewable, biodegradable, abundant availability, and cheap which could be a potential substitute of synthetic materials. Tensile strength of this bamboo is important requirements in various structural application. In this research, fiber fabrication process optimization of 3 types of local bamboo namely Gombong (Gigantochloa pseudoarundinacea), Haur Hejo (Bambusa tuldoides), and Tali (Gigantochloa apus) was investigated. These fibers were processed by varying the NaOH concentrations, immersion times, and immersion temperatures. The process parameters and its level variations of bamboo fiber fabrication were optimized using Taguchi method. Then the contribution of each process parameter was observed using the analysis of variance (ANOVA). From Taguchi method, the optimal parameter for highest tensile strength was obtained from Tali bamboo, indicated by 4% NaOH, 2 hours immersion time, and 60oC immersion temperature. From ANOVA, the contributions for each parameter are 70 % for bamboo type, 7 % for NaOH concentration, 4 % for immersion time, and 17 % for immersion temperature with error contribution of 2%. FTIR analysis was carried in order to observe chemical bonding in bamboos which were indicated Tali bamboo has larger lignin levels decreasing than Haur Hejo and Gombong.