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Phlebia sp. MG-60 as one of the white-rot fungi, which has an ability to produce ethanol from lignocellulosic biomass with a single process. This study aimed to determine the effects of nutrition addition and time of incubation for ethanol production, generated from lignocellulose degradation of OPEFB biomass by Phlebia sp. MG-60. By using a factorial experiment in a randomized block design, the effect of malt extract concentration and time of incubations were tested. The results revealed the addition of malt extract and time of incubations, as well as the interaction of both, were found to significantly influence the ethanol concentration (ρ value <0.05). The best treatment was obtained from the addition of malt extract 0.2 g/L, which released the highest amount of total reducing sugars, 26.489 mg/g during 15 days of incubations. Meanwhile, the lowest amount of lignin released 12.91%; weight loss 19.01% produced from 28 days incubation. Furthermore, the highest ethanol concentration (0.28%) was generated from the addition of malt extract 0.2 g/L for 5 days incubation.