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Cervical carcinoma is the second cancer type that common in woman and the most caused of woman death all over the world. Human Papilloma Virus (HPV) infection suggested could induced gene hypemethylation. Hypermethylation that occur on p16 tumor suppressor gene promoter resulted in silencing tumor suppressor gene so that the gene is inactive and contribute to malignancy of cancer cells. Tea paracytes or Loranthaceae known to be a plant that have an anticancer potency, correlated to its flavonoids content. Flavonoid from Scurrula atropurpurea (Blume) Danser (SAD) suggested have a role in the inhibiting methylation towards p16. This research objective was to identify the potency of Lawang - East Java Scurrula atropurpurea (Blume) Danser (SAD) flavonoids compound towards Hela cells. In this study, we examine flavonoids from SAD that been extracted in n-heksan, chloroform, etanol solvent, using TLC Chromatogram and LCMS. We also examined the cells proliferation using MTT assay, apoptotic level was examine by Tunnel, and then completed with methylation level detection using bisulfit direct sequencing method. The result shown that flavonoids that found were : flavanon, dihidroflavonol and flavon; dihidroflavonol, flavanon and cathecin, and flavonol, flavon, and EGCG, respectively.in n hexane, chloroform and ethanol fraction. Flavonoids that were found suggested to have proliferation inhibitor and apoptotic inductor potency via inhibition of p16 promoter of HeLa cells methylation. SAD flavonoids in the n-heksan and chloroform fraction shown the most potent in methylation inhibition. We suggested that combination of flavanon and dihidroflavonol have an important role in p16 demethylation. SAD flavonoids had activity to affect the methylation of p16 and bioactive compound could be one of a novel agent that have to be explore in the future as a demethylating agent in cancer.