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The challenges of 21st century globalization require students to have various skills needed in compete at the global level. One of the 21st century skills students need to have is critical thinking skills, but student-s critical thinking skills in Indonesia are still relatively low. To develop this skill, learning is needed along with appropriate assessment. The application of formative assessment in learning is one of the efforts in developing the skill. Through formative assessment, teacher can make appropriate learning strategies to facilitate student learning needs. However, the practice of assessment for learning rarely facilitates student learning needs. Thus, this study was needed for investigating the effect of formative assessment in develop critical thinking skill of students. The quasy experiment method is used in this study with the pretest posttest control group design. The experimental group was given the application of formative assessment through oral feedback during learning, while the control group was given the conventional assessment. The result showed that formative assessment through oral feedback can develop critical thinking skill of students, indicated by the experimental group has higher critical thinking skill improvement than control groups in Spermatophyta and Bryophyta chapter.