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ABSTRACT: Subcritical Assembly for Molybdenum Production (SAMOP) is a concept of nuclear reactor where the neutron multiplication factor (k-eff) less than one (k-eff ~ 0.99). Therefore, SAMOP reactor need an external neutron source for its operation. Uranyl Nitrate Hexahydrate, UO2(NO3)2or UN is used as fuel as well as target material for Mo-99 production.The Mo-99 isotope is used to generate Tc-99m which is the most widely radioisotopesused for diagnostic in nuclear medical fields. The composition of Uranyl Nitrate Hexahydrate (UNH)should be analyzed to achieve the required subcriticallevel in the SAMOP corei.e. k-eff ~ 0.99. Methodology for this analysis is a calculation of subcritical level of SAMOP by using WIMSDcomputer code. Using enrichment of uranium of 19.75% with 2.81 gr/cc of density, the composition shown 0.251724 for U and 0.748276 forN,O and H. The composition is then used as input for the neutronic analysis using the ORIGEN computer code as well as calculation of neutron source and thermal power of SAMOP. The result shows that the optimum k-eff is 0.9919 with the composition of U-235, U-238, N, O, H, U, N-O-H are 0.049715, 0.202009, 0.053448, 0.374138, 0.32069, 0.251724, and 0.748276 respectively.