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The limited information that is only in the form of text and images in the practicum guide that already exists causes various obstacles experienced by students in biology labs. The smartphone application comes with the potential to combine information in the form of text, images, videos and quizzes as a medium for student learning. This study aims to see the effect of the use of smartphone biology practical guide applications on the ability of science processes of students using quasi-experimental methods with pre-post-test control group design research design. The conclusion obtained is that the use of biological practicum guidance application has a positive influence on students scientific process abilities.