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Liquid waste with high amount of total suspended solid (TSS), is one of the significant environment issues from coal mining activities in Kalimantan island under high precipitation in of a very wide zone of coverage. The TSS concentration with Nano-sized particle in mine drainage caused by clay minerals which is dominated by montmorillonite type. Therefore, a huge chemical agent is usually used to treat TSS concentrations of the liquid waste less than 300 mg/L based on government regulation. However, in this case, the chemical flocculant triggers the onset of secondary pollutant because its carcinogenic and non-biodegradable. This study aims to observe specific fungi, Marasmius sp, as bio-flocculant for the treatment of mine drainage with extra high TSS. The fungi can produce biodegradable polymer that have ability to precipitate the montmorillonite with two mode action, they are bio-flocculant polymeration and mycelial trapping. A role model was running in laboratory scale less than 1 L in several graduated/measuring cylinders under 22-30 oC. The results of clarity test by using spectrophotometer shown a number of 86% clarity value averagely in one-hour treatment, while flocculation rate test indicated the amount of TSS concentrations became 110.1 mg/L. In this term, the minerals in the mine drainage samples from PT.BC mining excavation area, had been precipitated rapidly from previous measured TSS concentration at 11,000 mg/L.