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Lignin is an essential bio-polymer which is required in the formation of plant cell wall. The degradation of lignin in the nature is a major challenge due to the complexity of its structure. It has been long known that white rot fungi produce ligninolytic enzyme that can degrade lignin efficiently. To identify white rot fungi, screening test was carried out by testing different strains of Basidiomycota using czapek dox agar supplemented with Guaiacol (0,02%). The results showed that two out of thirteen strains of Basidiomycota have ligninolytic activity. In this study, we successfully obtained two strains that have ligninolytic activity. This study sheds light on further observation of these strains which potentially can be used for lignocellulose waste treatment especially oil palm empty fruit bunch (OPEFB).