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Seed vigour is more sensitive compared to the germination test and usually it is used to observe the ability of seed germination at a sub-optimum area and seed longevity. The variation among cultivar in seed vigour could be useful for the breeding program to develop a new cultivar. In this study, we used thirteen of new UNPAD sweetcorn lines and three SR lines to evaluate variation of seed vigour after storage among these lines. The results showed that these lines differentiated into two major groups using clustering analysis based on seed vigour (vigour index, electrical conductivity and normal dry weight seedling) parameters. The first group consisted of eight new UNPAD sweetcorn lines and the second groups consisted of five new UNPAD sweetcorn line and three SR lines. These result indicated that the new UNPAD sweetcorn line have a new genetic constituent. Besides, the result revealed that the new line which grouped with SR lines was higher on the vigour index and normal dry weight seedling parameter after 4 months storage than the other group by Students test. These result suggested that these lines could be useful for the breeding program to develop new cultivar with high seed vigour