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Cassava has been a premier commodity as the raw material for local-s food production. Gunungkidul regency well known as the central production of cassava, thus, become the main reference for the consumers i.e. persons, group, and firms. However, Cassava-s availability has remained a problem as its productivity cannot fulfill the demand of the market. Planting and harvest time are the premier problem as it is remain unclear, especially for local variety at Gunungkidul regency. This research aim to determine the proper time to plant and harvest for Gambyong varietycassava. Factorial experiment method is used in this research, which is arranged by Randomized Complete Block Design (RCBD). This research tested some a set of treatments on the planting times (September, October, November, and December), and the harvest age (four, five, six, seven, eight, and nine month). Each treatment is repeated three times, so that there are 72 treatment units that tested. The research result showed that the cassava planted on the September has growth, development, and tuber yield tend to increased started from 4 - 9 month of harvest age. Whereas, the cassava that planted on October up to December showed growth, development, and tuber yield tend to decreased on all harvest age.