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Abstract. Cattle breeding business need specific calf sex especially for fattening program needs of a male calf, while for dairy cows and breeding program are required for female calves. Therefore sexing technology is needed to get the calf sex as expected. The materials used were bull of Limousin semen with motility more than 70%. The sexing technology used was Percoll Gradient Centrifugation. This technology produces 83% of Y sperm in the upper layer, while 83% of sperm X in the lower layer so that each layer can be produced for sexing frozen semen. The sexed semen has been produced by Artificial Insemination Centre of Singosari Malang and has been marketed widely. The results showed that implementation of Artificial Insemination using frozen semen sexing (Sperm Y) could result in a male calf in the district of Tuban for 60 heads produced 61.5% pregnancy success in a single dose, whereas in double dose 63.6%. The male calf sex ratio of 42.11% in a single dose and 78.95% in double dose.