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Biodiesel is one of the most promising renewable alternative fuels. Subcritical water is a green technology which can be employed to convert rice bran with water and methanol to fatty acid methyl ester (FAMEs) or biodiesel with adding CO2 gas as co-solvent and N2.This study investigates the influence of operation condition to obtain biodiesel from rice bran oil. Some solvents concentrated, range of temperature ,pressurized gas and reaction time were applied in this research. Rice bran, water, and ethanol were put into subcritical water reactor in a certain ratio at 40 bar to carried out the reaction under subcritical condition. The best condition 30/10 methanol-water (v/v), t= 7 h, T = 200 0C,CO2 as co-solvent were highest yield oil obtained 18% with oleic acid methyl ester as the major identified biodiesel