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This study aims to the generation of teaching E-LITE-S (Electronics Literacy Science) to the matter interaction living organism and environmental being used to be improvement science literacy students. The research is research of development by adopt the four-D which are (1) define covering analysis matter, analysis the concept, and analysis indicators; (2) design include the development of grating about, quiz, and the prototype of teaching materials E-LITE-S (3) the development of covering test validation the people of, assessment users E-LITE-S, and a pilot limited. Data were collected with this validation experts and assessment teachers, test literasi science, and chief response students. Data analyzed a sort of descriptive set quantitative. The result showed material get the excellent of the namely 97 %, the CVR and CVI of 3,56 and 0,87. These tests science literacy students before use of 60,66 material and after using material of 76,47, N-Gain literacy students at 40,19 science, and some 91 % students are very agree with material E-LITE-S.