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This study aims to determine factors that cause learning difficulties to anatomy and physiology subjects lesson by class X student cosmetology Programs at SMK Negeri 3 Tangerang in Academic Year 2016 with a survey method. The populations were 64 people from class X students and Data collection tool used is questionnaires. The trial questionnaires carried out by 25 class XI cosmetology respondents. Instrument analysis carried out on validity and reliability, with 40 statement items and reliability result r11 = 0.954. The results of this study showed that the factors affecting students difficulties mainly on internal factors that are motivational factors indicators with sub student 88% learning persistence factor and independent learning factor equal to 94%. While external factors are indicators of teacher attention factor, with teachers sub-factor appreciation to students difficulties by 84%, and the factor of teachers involvement in students activities is equal to 91%, showing that any increase in the value of a teacher attention every 101,286 can cause an 879 increase with learning difficulties showed the 6.1% test effect, coefficient determination while the remaining 93.9% is influenced by other factors.