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In general, chicken slaughterhouses have capacity of slaughtering more than 40.000 chickens per day so it generates a high wastewater. One of appropriate treatments is Granular Aerobic Sludge in Sequencing Batch Reactor (GAS-SBR) since wastewater from chicken slaughterhouse has high BOD and COD in fluctuation. The purpose of this research is to analyze the reactor system ability to decrease parameters of BOD, COD, TSS, and ammonia on wastewater. In this research, the concentration variations were 6406.4 mg/L (100%) as initial concentration, 4804,8 mg/L (75%) and 2303,2 mg/L (50%). With 4 hour aeration time, the highest BOD removal with 100% load is 56.25% and an effluent is 1400 mg/L; COD is 75% removed with effluent of 768 mg/L at 50% load; TSS is 36.73% removed with effluent 1.79 mg/L at 100% load; while for Ammonia is 67.91% with 1.11 mg/L at 75% load. The parameters of TSS and ammonia have meet the quality standard of Environmental Ministry Decree no.5, 2014 for slaughterhouses wastewater, while BOD and COD parameters are steel exceeding the standards. The GAS-SBR could remove BOD and TSS parameters from highest concentration load, while ammonia and COD parameters could remove in influent concentration load diluted.