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The research was aim to determine the effect of urea fertilizer and biofertilizer sources on the physiological and agronomic characteristics of Aloe vera L. plants in coastal sandy soil. The research was arranged in a factorial Randomized Complete Block Design three replications. The first factor was the dosage of urea fertilizer which is 300 and 450 kg per ha. The second factor was the source of the biofertiliser, namely Rhizobacteria of bamboo, Rhizobacteria of gamal, Rhizobacteria of scrubs, Rhizobacteria of peanuts, Rhizobacteria of bamboo/PGPR, PGPR, Rhizobacteria of bamboo/Mycorhiza and Mycorhiza. Observation variables include index, density, length of porous stomata, width of porous stomata, total chlorophyll concentration, ANR, proline concentration, growth and yield of leaf. Analysis of results using variance, followed by DMRT at a significant level of 5%. The results showed that there was an interaction between the urea fertilizer and the biofertilizer source on all observation variables. The combination of 300 kg urea fertilizer and bamboo rhizobacteria has an effect on physiological characteristics, while the combination with PGPR or mycorhiza increases the growth and yield of aloe vera leaves.