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Indigofera zollingeriana is a feed that has high crude fiber content so it is difficult to use for poultry feed. The fermentation process using fungus is expected to overcome this problem. The study aimed to determine the interaction between dose and fungi kinds, with the length of fermentation on the protein and crude fiber content of I. zollingeriana. Another goal is to determine the optimum conditions for fermenting I. zollingeriana. The method used a Completely Randomized Design (CRD) factorial pattern. The first factor consisted of 6 levels of the different fungi dosages, namely 0.1%, 0.2% and 0.3% Trichoderma harzianum; 0.1%, 0.2% and 0.3% Aspergillus niger. The second factor was four levels of the duration of fermentation: 24 hours, 48 hours, 72 hours and 96 hours. All treatments were repeated twice. The experimental data were tested by F test, then Duncans analysis was carried out. The results showed that there was an interaction between dose and fungi kinds with the length of fermentation. The optimal condition for fermenting I. zollingeriana with T. harzianum fungus was at a dose of 0.3% and 96 hours fermentation time. This treatment gave the increasing of protein content and reducing crude fiber content.