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Analysis Antifungal Compounds Produced By Endophytic Bacteria From Oil Palm Plant Using Bioautography
Emilia Candrawati*1, Bedah Rupaedah2, Sumpono3, Agus Sundaryono3


1 Science Education Study Program, University of Bengkulu, Kandang Limun, Bengkulu
2 Biotechnology Center, Agency for Assessment and Application of Technology, Serpong, South Tangerang, Indonesia
3 Graduate School of Science Education, University of Bengkulu, Kandang Limun, Bengkulu


Abstract

This study was undertaken to evaluate the antifungal activities of the bioactive compounds produced by endophytic bacteria isolated from oil palm plants using TLC-Bioautography. Antifungal compounds used in this study have been extracted from potential endophytic bacteria from previous studies using ethyl acetate solvents. The antifungal compound was tested for its activity against the pathogenic fungus Fusarium oxysporum f.sp by using the TLC-bioautography method. The results showed 3 spots on the TLC plate for samples of B11 antifungal compounds with an incubation time of 54 hours and B11 with an incubation time of 24 hours. Observations of bioautographic antifungal activity test showed the presence of inhibition zones with the largest average percentage given by B11 with an incubation time of 24 hours is 11.59%. The inhibition zone provided by B11 with an incubation time of 54 hours is only 11.08%.

Keywords: Endophytic bacteria, Bioactive compound, Bioautography, Fusarium oxysporum f.sp.

Topic: Other Relevant Topic

Link: https://ifory.id/abstract/Fd4kqRQeKHEy

Conference: Mathematics and Science Education International Seminar (MASEIS 2019)

Plain Format | Corresponding Author (Emilia Candrawati)

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