Indonesia Conference Directory

Stunting is a growth disorder in the form of linear growth retardation. Risk factors, both internal and external that occur from the time of pregnancy to the age of two years greatly influence the occurrence of stunting. Rotenone is a natural pesticide that proved had stunting effect on zebrafish. The mechanism of action of rotenone is by inhibiting mitochondrial complex I, thus increase the production of reactive oxygen species (ROS) and ATP depletion. Folic acid not only as micronutrients needed in growth , but also have an activity. The purpose of this study was to determine the effect of folic acid on body length, heart rate, motility and bone ossification of rotenone-induced zebrafish larvae stunting model. Rotenone and folic acid administered from 2 -72 hpf (hour post fertilization). The concentration of folic acid used in this study was 50, 70, and 100 μM, while rotenone 12.5 ppm. Statistical analysis using ANOVA showed a significant difference in body length, heart rate, motility and bone ossification among the rotenone and folic acid group. Folic acid concentrations of 50 and 70 μM could normalize the body length, heart rate, whereas folic acid 100 μM actually increases bone ossification. It can be concluded that folic acid could protect stunting in a dose-dependent manner.