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Research on the manufacture of polyurethane with chitosan and bentonite fillers modified with CTAB into montmorillonite (MMT) which will be applied to medical devices. The raw polyurethane from castor oil and diisocyanate is in the form of Toluene Diisocyanate (TDI). The addition of chitosan to castor oil polyurethane functions as an anti-bacterial agent. Pure polyurethane still has a deficiency of high temperatures and therefore added MMT which functions as a heat-retaining agent. The bacteria used are E. coli bacteria and Staphylococcus Aureus bacteria. The results showed a polyurethane that can withstand high temperatures is PU-MMT-CS 6%. The best anti-bacterial properties were obtained in polyurethane containing chitosan with higher concentrations (PU-MMT-CS 6%).