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In Vitro Multification of Dendrobium sp with Plant Growth Regulator
Mislah S Harahap (1), Fauziyah Harahap (2*), Syahmi Edi (2) and Rosmayati (3)

(1 )Biology Education of Postgraduate school Universitas Negeri Medan, Jalan Willem Iskandar, Pasar V Medan Estate, North Sumatera 20221, Indonesia
(2)Departement of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Medan, Jalan Willem Iskandar, Pasar V, North Sumatera 20221, Indonesia *fauziyahharahap[at]gmail.com
(3)Departement of Biology, Faculty of Mathematics and Natural Science, Universitas Sumatera Utara, Jalan Bioteknologi No.1, North Sumatera 20155, Indonesia


Abstract

Orchids are plants that has aesthetic value and high economic important. Included in to ornamental plant whose existence is diminishing and endangered. Therefore, to preserve this plant it is necessary to plant one way through in vitro techniques. The aim of this study was to determine the best interaction of growth regulator Benzyl amino purine, coconut water and the interaction of both multiplication of Dendrobium sp. The research method used a completely randome design with various concentrations of Benzyl amino purine (BAP; 0,1,2,3 mg/L) and coconut water concentrations (0%,5%,10%). Were the observation parameter are number of shoots, leaves and roots. Data were analyzed descriptively and inferentially with ANOVA. The results shows the real effect of BAP interaction, between coconut water shoots and leaves, but did not significantly affect the number of roots. Within 12 weeks of observation the highest number of shoots and leaf obtained from a combination of 10% coconut water + BAP 3 mg/L were 12.00 and 5.00. The lowest number of shoots and leaves was obtained in combination of 5% coconut water + BAP 1 mg/L, 6.00 and 1.00.

Keywords: Benzyl amino purine, Coconut water, Dendrobium sp., In vitro

Topic: Biochemistry, Biotechnology and Pharmacy

Link: https://ifory.id/abstract/HvYTtdXD9jzR

Conference: International Conference on Science and Technology Applications (ICOSTA 2019)

Plain Format | Corresponding Author (mislah sahila harahap)

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