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The need for energy use is increasing along with population growth, increasing energy consumption by the community, and due to the use of various types of equipment to support comfort. Biogas production contributes as a sustainable renewable alternative energy because raw materials are easily obtained and economical. However, biogas emissions can cause damage to the environment due to the presence of hydrogen sulfide pollutants. One method that can be used for this separation is adsorption using laterite soil. The advantage of using laterite soil as an adsorbent is that it is cheap, easy to obtain, and adsorption can occur at room temperature. In this study, the parameters to be varied are particle size and mass of laterite soil used. Before use, the laterite soil is dried first at 100oC for 1 hour. The process of separating hydrogen sulfide from biogas was carried out by flowing biogas into the adsorption column with a diameter of 2.5 cm containing adsorbent of laterite soil. Variations in the experiment are particle size, 6 and 21 mesh, and bed mass is 30 and 50 grams. The biogas flow rate is kept constant at 1.5 liters / minute. The particle size and bed mass affect the performance of adsorption. Reduction of particle size of the adsorbent from 6 to 21 mesh caused the hydrogen sulfide adsorption capacity to increase to 2.13 times. The bed mass addition from 30 g to 50 g caused the hydrogen sulfide adsorption capacity to increase to 1.15 times. The adsorbent worked optimally when the particle size was 21 mesh and with a mass of 50 g which had hydrogen sulfide adsorption capacity up to 14.96 mg H2S / g adsorbent.