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Bacillus subtilis phage is a virus that specifically infects Bacillus subtilis bacteria. The existence and position of B. subtilis phage are very important related to the dynamics of ecosystem balance in nature and its applications in the agricultural industry, especially phage therapy and biocontrol in overcoming contamination of raw materials and processing equipment of agricultural products that are environmentally friendly. Comparative study of morphology and genome size of B. subtilis phage was carried out to understand and determine of phage interactions with their hosts and the characteristics of gene products possessed by each phage. Until this article is presented, it is known that there are 18 species of Bacillus subtilis phage from various regions of the world. Based on the morphological structure, there are 9 species in the Myoviridae family, 4 species in the Siphoviridae family, and 5 species in the Podoviridae family. The genome size of B. subtilis phage ranges is known to range from 18,000 to 160,000 bp with ORFs different ranges 15–250, which can be identified their protein function as between 20–60%.