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The purpose of this study are: (1) Knowing and analyzing the influence of quality service and customer satisfaction on patient loyalty; (2) Knowing and analyzing the influence of customer quality on patient loyalty; (3) Knowing and analyzing the effect of customer satisfaction on patient loyalty at the PKU Primary Clinic Muhammadiyah Cangkringan. This study uses a quantitative approach. The research variables included quality service and patient satisfaction as independent variables, and patient loyalty as the dependent variable. Population of this study was visitors in all service units of the Pratama Clinic PKU Muhammadiyah Cangkringan in 2016 amounting to 5,283. About 100 people were obtained as samples used the Slovin technique and 4 informants were interviewed to complete quantitative data. The type of data used is primary and secondary data. Data collection techniques consist of questionnaires supported by interviews. Validity and reliability test used the Correlation Product Moment test, and Crobench Alpha. Descriptive data analysis techniques used SPSS for windows version 15.0 and Structural Equation Modeling (SEM) analysis. The results of the study indicate that Quality of service consisting of five dimensions, namely; trustworthy (responsiveness), guarantee of service quality (asurance), customer empathy (empathy) and physical evidence (tangible) and patient satisfaction that cover three dimensions: product quality meets expectations; quality of service meets expectations ; the price is as expected, simultaneously has a positive effect significantly on patient loyalty. Simultaneous and positive effects of both service quality variables and Customer Satisfaction (0.689); Quality Service (X1) has a positive effect significantly (0,928) to patient loyalty at the PKU Pratama Clinic Muhamadiyah Cangkringan; Customer satisfaction which includes dimensions: product quality meets expectations, service quality meets expectations, and prices according to expectations also provide a positive effect significantly (1,167).