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Abstract. Changes in pH, temperature, and salinity in seawater can indicate the occurrence of ocean acidification. This study aims to study changes in acidity that occur in Ohoililir waters, Southeast Maluku, which is part of the Banda Sea. This research was conducted in March 2019 as a study of the prefix of oceanic equities in the Southeast Maluku region which is part of the Banda Sea which is currently one of the priority researches in Indonesia, and sampling at 10 points associated with coral reefs and seagrass ecosystems. The results obtained were pH ranging from 8.567 – 8.851 with an average of 8.6879, temperatures ranged from 29.285 – 30.281oC with an average of 29,7412°C, and salinity ranged from 33.041-33.33 with an average of 33,2355. Its shows that pH, temperature, and salinity of Ohoililir waters are still a good range for the life of marine biota.The correlation between pH and temperature is -0.097 and the correlation between pH and salinity is in the range of -0.054 which indicates has no relationship between pH and temperature and salinity. Beside that, pH has negative correlation to temperature and salinity. This shows the need for further research on the relationship between these three or more parameters to see the effect on ocean acidification.