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Feeder cells are inactivated cells that can not proliferate but have capability to produce growth factors and extracellular matrix for attachment and development of the cell culture. Feeder cells are widely used to support pluripotent stem cell (PSC) cultures which is predicted as one of the future cell therapy. One of the type of PSC is embryonic stem cells. Some of the feeder type which already used in stem cells propagation are mouse embryonic fibroblasts (MEF), rat embryonic fibroblast (REF), cumulus cells and mesenchymal stem cells (MSC). Research using feeder cells can be utilized to analyze various growth factors secreted by this cells that can lead to development of synthetic production of these substances. This paper will discuss REF cell culture by explant method and the inactivation process of REF to be used as feeder cells.