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Plant sound wave technology (PSWT) is the use of sound waves to improve crop productivity and quality through optimization of the opening of stomata so that plants are able to absorb nutrients and water optimally. The purpose of this study is to test PSWT to increase the vegetative growth of mustard greens (Brassica juncea L.). The type of music used in PSWT is Javanese gamelan entitled Puspawarna. PSWT exposure time is given to plants in the morning and evening. The frequencies used are 3-5 kHz, 7-9 kHz, and 11-13 kHz. The mixture of organic planting media i.e. roasted husk, cocopeat, and moss with a ratio of 2: 2: 1 for soil, organic growing media and organic fertilizer. Observations were made on gap of plant height, plant height, plant length, wet weight, stomata opening, leaf chlorophyll, leaf area, and number of plant leaves. The results showed that the frequency and the mixture of organic planting media significantly affected the wet weight, plant length, stomata opening, plant height, leaf chlorophyll, leaf area, and number of leaves. The best PSWT method for growing mustard greens is using frequency of 3-5 kHz and and moss as the organic planting media. The maximum yield of vegetative growth resulted in the most optimal stomata openings of 70.84 µm – 159.46 µm, with plant height of 19.47 cm, plant length of 29.47 cm, leaf area of 31.99 cm2, the number of leaves is 5.33 strands, leaf chlorophyll of 31.60 cci, and wet weight of 5.01 g.