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The existence of organic waste is believed to be the cause of changes in the physical and chemical parameters of the river environment, which have an impact on the diversity of macrozoobenthos in it. However, not many studies have revealed what variables are highly correlated with macrozoobenthos diversity. This study aims to determine the impact of shrimp pond waste inputs and the variables that most correlate with macrozoobenthos diversity. This quantitative descriptive study uses purposive random sampling technique. Sampling was carried out at five stations along the Kali Jeruk - Trenggalek, East Java stream with three repetitions. The identification of macrozoobenthos was carried out in the Fisheries Laboratory while testing physical and chemical parameters was carried out in the laboratory of Perum Jasa Tirta - Malang, East Java. The diversity of macrozoobenthos was analyzed using test principal component analysis (PCA). The results showed that macrozoobenthos were found in ten species from three classes, seven families, and nine genera. Meanwhile, the parameters that correlate most strongly with macrozoobenthos diversity are dissolved oxygen (DO).