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The need for high nutritional fruits such as melon continues to increase in Indonesia. Melon also becomes a source of income because it has marketing prospects and high interest from the society. Begomovirus is one of severe disease which often cause a decrease of fruit production. Until now there were only two cultivars which were claimed to have resistance against Geminivirus, ‘Gracia- and ‘Madesta-. Furthermore, the Faculty of Biology UGM has developed several varieties of melon which was expected to become resistance against Begomovirus. The aims of this research was evaluate the resistance of 9 melon genotypes to Begomovirus infection using symptoms observations and molecular detection. Melon samples was inoculated by insect transmission inside screenhouse and observed every 3 days for 4 weeks. Symptoms severity was revealed as vulnerability index. Furthermore, the results of symptom observations was confirmed using molecular detection. The results showed that no cultivars was truly resistance against Begomovirus. ‘Gracia-. ‘Tacapa GB-, and ‘Tacapa Silver- showed tolerance response against Begomovirus while the others only categorized as moderate susceptible. All infected samples which identified by symptoms observations also amplified DNA virus when tested using PCR. Molecular detection was promising fast and reliable detection for Begomovirus infection.