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The purpose of this study was to found the distribution of Pyricularia oryzae races in three different regencies in South Sulawesi as well as gene analyze linked to the virulence character of P. oryzae in causing blast disease on rice. Determination of P. oryzae races was conducted using 1 set of rice differential varieties consisting of Asahan, Cisokan, IR 64, Krueng Aceh, Cisadane, Cisanggarung and Kencana Bali. Intensity of blast disease was observed seven days after inoculation using the IRRI evaluation standard using a 5-9 scale of necrotic area to determine the host resistance level. Molecular characterization of P. oryzae isolates code for their virulence characters was performed using primer Erg2 (1440 bp), Pwl2 (900 bp), and Cut1 (1730bp). The amplified DNA band appear for each primer of each isolate was evaluated with a value of 1 if present and 0 (absent). Out of 20 isolates were successfully collected: from Bone regency 8 isolates, from Maros regency were 8 isolates and from Gowa regency were 4 isolates. Inoculation of P. oryzae isolates on 7 differential rice varieties showed, that 12 races (000, 001,003,010, 020,023, 100, 102,110,111,173, and 251) were exist between P. oryzae isolates tested. However, the 020 race was dominant in all district. Using 3 primers coded for virulence of 10 P. oryzae isolates, only three haplotypes were found. There were haplotypes C-011, E-010, and F-110. Haplotype C-011 were most dominant (8 isolates), whereas haplotype E-010 and F-110 were present only in each one isolate respectively.