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The BNCT in-vitro test using a microtube irradiated on the neutron beam of the RSG-GAS beam tubes has been simulated. The simulation was performed using a PHITS computer code by modeling a microtube containing boron solution which was placed 5 cm in front of the beam tubes. Inside the beam tube was installed a beam shaping assembly (BSA) using 80 cm of MgF2 as a filter to reduce fast neutron and gamma fluences. Microtube containing 1.5 mL of boron solution with boron enriched 10B of 90%. The microtube dose i.e alpha dose, 7Li dose, proton dose, and gamma dose were calculated for each variation of boron concentration from 0.1 - 20 ppm. The simulation of microtube containing 1 ppm boron solution shown that irradiation of BSA-S5 neutron beam can be produced at a dose of 20 Gy with the shortest irradiation time of 2.5 hours.