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Background : Job stress arising from the work it receives can have an impact on ones performance. One of the work stresses that occur in anesthetist nurses can have an impact on work negligence, such as a medication error, a medication dosage error. Negligence caused in work in the operating room can have a serious impact on the patient, so that there will be unwanted events (KTD), near-injury events (KNC), potential injury events (KPC) and non-injury events (KTC). The things that cause work stress are the workload that they receive, the amount of salary earned, the rewards obtained from their performance, and the encouragement from within themselves and from outside to improve their performance. Purpose : To find the factors that effect the stress of anesthetist nurses work in the operating room. Methods : This study used an observational analytic method with a cross sectional design. This research was conducted in February - April 2019. The sample of this study was 32 respondents with quota sampling technique. The results showed that there was an effect of workload, reward and motivation on work stress with. And there is no effect of work period and educational on work stress. Conclusion : There is an effect of workload, reward, and motivation on the stress of anesthetist nurse work in the operating room. There was no effect on work period and education on the stress of anesthetist nurses work in the operating room.