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This study aims to obtain information and to analyze the biological - mathematical attitudes of students in quantitative literacy- based ecosystem instruction. The research used a quasi-experimental design with 131 six grade students in two high school.. The Biological - mathematical attitude was obtained with the Math-Biology Values Instrument (MBVI) questionnaire and quantitative literacy was asses with multiple choice questions. The results shows that majority of students feel interested, feel the benefits and feel the importance of mathematical abilities in solving biological problems, and almost all students do not feel difficulties with mathematics in ecosystems instruction. Student very high interpretation and calculation abilities as well as high representation and analysis skills.