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The army worm, Spodoptera litura, is the major leaf feeding-pest of soybean in Indonesia. The aim of the study was to identify the resistance of soybean genotypes to the army worm based on the preference index. A total of 150 soybean genotypes were grown under field condition from February to May 2018 in Malang (East Java, Indonesia). The preference test was conducted in the laboratory, and using G100H as resistant check to army worm. The result showed that the preference index of 150 genotypes was ranged from 0.09-1.82. A total of 105 genotypes were less preferred by larvae of S. litura (resistant), 39 genotypes were more preferred by larvae of S. litura (susceptible), and a genotype has equal resistant with G100H. Based on the preference index and morphophysiological characters, 150 soybean genotypes can be grouped into eight clusters. A genotype (Anjasmoro/Rajabasa-41) has characteristics of low leaf moisture content, dense leaf trichomes, and the preference index of 0.14. Those characters could be the attributes of the soybean genotypes resistant to S. litura. The Anjasmoro/Rajabasa-41 has the opportunity to be developed in tropical regions of Indonesia or can be used as a source of resistance genes to S. litura.