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Hypercholesterol is caused due to consumption of carobohydrate and high fat so causing oxidative stress results in the formation of Glycation End-Products (AGE). This study aimed to determine the effect of giving single garlic extract on the levels of Advanced glycation end products (AGE) and Receptor of Advanced glycation end products (RAGE) in the epididymis of mice (Mus musculus) Balb / C strain of a high fat and carbohydrate diet models. The mice were acclimatized for a week and then were given HFD for 6 weeks. This research is an experimental study using Randomized Block Design (RBD) with 6 treatments and 4 repetitions. This study requires a total of 24 mice. The treatment group was divided into 6 groups, namely the normal group (N), the negative control group (K-), the positive control group (K +), and the treatment group by administering a single garlic extract with various doses (125 mg / Kg BB, 250 mg / Kg BB, and 500 mg / Kg BB). Measurement of AGE levels in the epididymis of mice on high-fat and carbohydrate diet models was carried out by the ELISA method after 45 days of treatment. The measurement results of AGE in the epididmis have a significant effect on the decrease in AGE in the epididymis according to the ANOVA test conducted (0.003), the results of a single ANOVA analysis of significant RAGE levels <0.05 (0.0). Giving a single garlic extract (Allium sativum L.) had a significant effect. Positive control group (K +) and single garlic extract dose 250 mg / Kg BB (P2) were optimal treatments for reducing RAGE levels in the epididymis of mice (Mus musculus) Balb / C strains on high-carbohydrate and fat diets