Indonesia Conference Directory

Chlorella vulgaris and Navicula salinicola have been reported to have high lipid content and are potential to be used as an alternative source of bioenergy precursor compounds. However, to obtain a high biomass and lipid with autotropic cultivation condition is still a challenge therefore other cultivation condition such as photoheterotropic need to be explored. In addition, the influence of cultivation conditions on the production of biomass and lipid for diatomic microalgae, especially Navicula salinicola, is still rare explored. This study aims to analyze the effect of organic carbon sources namely sugarcane addition on the production of biomass and lipid of Chlorella vulgaris and Navicula salinicola. Analysis of biomass concentrations is evaluated by measuring dry cell weight (DCW), while lipid concentrations is evaluated by measuring total lipid levels from the extraction results using the Folch method. Lipid concentrations is calculated based on total lipid levels and biomass concentration obtained. The profile of fatty acid methyl ester (FAME) is analysed by using GC-MS. Based on the results of this study, it was observed that addition of sugarcane as an organic carbon source has increased the biomass production in Chlorella vulgaris and Navicula salinicola by 50.10% and 69.40% respectively than those are cultivated without the addition of sugarcane. Similarly, the addition of sugarcane has been showed to induced lipid accumulation in Chlorella vulgaris and Navicula salinicola by 34,45% and 25,51% respectively compared to those are cultivated with carbon source from the air. On the other hand, the addition of sugarcane as an organic carbon has decreased the variations of fatty acid in Chlorella vulgaris and Navicula salinicola by 3 and 4 types respectively than those are cultivated without the addition of sugarcane. The addition of sugarcane can suppress the variations of fatty acids in Chlorella vulgaris and Navicula salinicola but it stimulates the synthesis of palmitic acid (C16:0) which has potential to be applied to the biodiesel industry.