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Nano-hydroxyapatite had been synthesized by the basic ingredients of limestone using an alkaline precipitation method. Hydroxypatite synthesis was done by using H3PO4 0.3M solvent as a source of phosphate and NH4OH 1M as pH controller. The hydroxyapatite powder obtained was characterized by XRD, SEM and FTIR. The XRD test was done to determine the constituent compounds and the crystalline hydroxyapatite that had been made. From the results of the characterization of XRD powder had been formed into Calcium phosphate hydroxide (Ca10H3O27P6) compound or hydroxyapatite with hexagonal crystal form. The nanocrystalline size of the limestone hydroxyapatite powder was 66.05 nm. The SEM test was done to determine the pore size and Ca/p ratio of the limestone hydroxyapatite that had been made. From the SEM morphology test, the pore size obtained was 64.43 nm and the Ca /p ratio was 1.25.