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This study aims to determine the physical and chemical quality of oil palm midrib compost with the addition of ruminant rumen bioactivators (cattle, goats and buffalo). The material used in this research is palm oil fronds and ruminant animal rumen obtained at Gandus Abattoir in South Sumatra. This study uses a Completely Randomized Design with 5 treatments with 4 replications consisting of P0 (palm frond compost without adding bioactivators), P1 (palm frond compost with bio4 activator EM4), P2 (palm frond compost with bio rumen liquid bioactivator), P3 (Compost of oil palm fronds with buffalo rumen liquid bioactivator), and P4 (Compost of oil palm fronds with goat rumen fluid bioactivator). The parameters measured in the study are physical quality (odor, texture, pH, temperature and color) and chemical quality (nitrogen, water content, phosphorus, calcium and C-organic). The data obtained were analyzed by ANOVA, if there were real differences followed by Duncans test. the results show that the compost of oil palm fronds using rumen bioactivators from buffalo (P4) has better quality compared to EM4 bioactivators (P1) or bioactivators from goat (P2or cow rumen (P3).